Article successfully added.

CyFlow™ CD4 PE

CyFlow™ CD4 PE
Alternative Name: Leu3a, T4
Antibody: Yes
Antigen: CD4
Application: Flow cytometry
Clonality: monoclonal
Clone: OX-35
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: PE
Isotype: IgG2a
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Rat
Product number: BU785433

For Research Use Only

$300.26 CAD*

Price excludes any applicable taxes plus shipping costs

Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD4 PE

Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenMLR generated rat Th cells
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Dragun D, Lukitsch I, Tullius SG, Qun Y, Park JK, Schneider W, Luft FC, Haller H: Inhibition of intercellular adhesion molecule‑1 with antisense deoxynucleotides prolongs renal isograft survival in the rat. Kidney Int. 1998 Dec; 54(6):2113‑22. < PMID: 9853277 > | Salomon I, Netzer N, Wildbaum G, Schif-Zuck S, Maor G, Karin N: Targeting the function of IFN‑gamma‑inducible protein 10 suppresses ongoing adjuvant arthritis. J Immunol. 2002 Sep 1; 169(5):2685‑93. < PMID: 12193742 > | Tsuji H, Kawaguchi S, Wada T, Nagoya S, Inobe M, Yamashita T, Ishii S, Uede T: Adenovirus‑mediated in vivo B7‑1 gene transfer induces anti‑tumor immunity against pre‑established primary tumor and pulmonary metastasis of rat osteosarcoma. Cancer Gene Ther. 2002 Sep; 9(9):747‑55. < PMID: 12189524 > | Yan Y, Devos T, Yu L, Xia G, Rutgeerts O, Goebels J, Segers C, Lin Y, Vandeputte M, Waer M: Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J Immunol. 2003 Jun 15; 170(12):5936‑46. < PMID: 12794120 > | Baba T, Ishizu A, Iwasaki S, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M: CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype. Blood. 2006 Mar 1; 107(5):2004‑12. < PMID: 16269616 > | Gelderman KA, Hultqvist M, Holmberg J, Olofsson P, Holmdahl R: T cell surface redox levels determine T cell reactivity and arthritis susceptibility. Proc Natl Acad Sci USA. 2006 Aug 22; 103(34):12831‑6. < PMID: 16908843 > | Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D‑ and granzyme/perforin‑dependent mechanism. J Immunol. 2008 Mar 1; 180(5):2999‑3006. < PMID: 18292522 > | Ramiro-Puig E, Pérez-Cano FJ, Ramos-Romero S, Pérez-Berezo T, Castellote C, Permanyer J, Franch A, Izquierdo-Pulido M, Castell M: Intestinal immune system of young rats influenced by cocoa‑enriched diet. J Nutr Biochem. 2008 Aug; 19(8):555‑65. < PMID: 18061430 > | Hishikari K, Suzuki J, Ogawa M, Isobe K, Takahashi T, Onishi M, Takayama K, Isobe M: Pharmacological activation of the prostaglandin E2 receptor EP4 improves cardiacfunction after myocardial ischaemia/reperfusion injury. Cardiovasc Res. 2009 Jan 1; 81(1):123‑32. < PMID: 18805784 > | Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am J Physiol Heart Circ Physiol. 2010 Mar; 298(3):H938‑44. < PMID: 20044442 > | Monzon-Casanova E, Steiniger B, Schweigle S, Clemen H, Zdzieblo D, Starick L, Müller I, Wang CR, Rhost S, Cardell S, Pyz E, Herrmann T: CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS One. 2010 Sep 30; 5(9):pii:e13089. < PMID: 20927351 >