CyFlow™ CD3 PE-Cy5

CyFlow™ CD3 PE-Cy5
Laser: Blue, Green, Yellow
Target Species: Human, Non-Human Primates
Antigen: CD3
Field of Interest: Immunophenotyping
Clone: UCHT1
Regulatory Status: RUO
Alternative Name: Leu4, T3
Application: Flow cytometry
Format/Fluorochrome: PE-Cy5
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Product number: BE142793

For Research Use Only

$335.58 CAD*

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HLDA Workshop HLDA I—WS Code T 3 HLDA III—WS Code T 126 HLDA III—WS Code T 471 HLDA VI—WS... more
CyFlow™ CD3 PE-Cy5
HLDA WorkshopHLDA I—WS Code T 3 HLDA III—WS Code T 126 HLDA III—WS Code T 471 HLDA VI—WS Code T 6T-CD3.1
Quantity100 tests
ImmunogenHuman thymocytes followed by Sezary T cells
SpecifityThe mouse monoclonal antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the ε chain of the CD3 complex. The monoclonal antibodies UCHT1 and SK7 recognize overlapping epitopes.
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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