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CyFlow™ CD4 Biotin

CyFlow™ CD4 Biotin
Target Species: Human
Antigen: CD4
Field of Interest: Immunophenotyping
Clone: MEM-241
Regulatory Status: RUO
Alternative Name: Leu3a, T4
Application: Flow cytometry
Format/Fluorochrome: Biotin
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: CY951579

For Research Use Only

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HLDA Workshop HCDM (HLDA VIII)—WS Code M241 Concentration 1 mg/mL Quantity 0.1 mg... more
CyFlow™ CD4 Biotin
HLDA WorkshopHCDM (HLDA VIII)—WS Code M241
Concentration1 mg/mL
Quantity0.1 mg
Volume0.1 mL
Immunogen2 N-terminal domains of human CD4 fused to human IgG1 Fc
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 0.7·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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