CyFlow? CD4 APC

CyFlow? CD4 APC
Alternative Name: Leu3a, T4
Antigen: CD4
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-241
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CZ151448

For Research Use Only

$300.26 CAD*

Price excludes any applicable taxes plus shipping costs

Build Your Own Reagent Panel
HLDA Workshop HCDM (HLDA VIII)—WS Code M241 Quantity 100 tests Volume 1.0 mL... more
CyFlow? CD4 APC
HLDA WorkshopHCDM (HLDA VIII)—WS Code M241
Quantity100 tests
Volume1.0 mL
Immunogen2 N-terminal domains of human CD4 fused to human IgG1 Fc
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Available Technical Data Sheets

Specific References

| Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent?resistant T?cell membrane microdomains. Tissue?Antigens. 1999?Jan; 53(1):33?40. | Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc?Natl?Acad?Sci?USA. 2002?Feb?19; 99(4):2008?13. | Clapham PR, McKnight A: Cell surface receptors, virus entry and tropism of primate lentiviruses. . J?Gen?Virol. 2002?Aug; 83(8):1809?29. | Brdickov? N, Brdicka T, Angelisov? P, Horv?th O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejs? V: LIME: a new membrane Raft?associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J?Exp?Med. 2003?Nov?17; 198(10):1453?62. | Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006: human cell differentiation molecules. J?Immunol?Methods. 2007?Jan?30; 319(1?2):1?5. | Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus?infected patients in Zimbabwe. Clin?Vaccine?Immunol. 2007?Mar; 14(3):293?8. | Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of na?ve and memory CD4+ T cells by alternatively activated dendritic cells. J?Leukoc?Biol. 2008?Jul; 84(1):124?33. | Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture?free differentiation in M?CSF and IL?3. Exp?Hematol. 2008?Sep; 36(9):1167?75. | Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL?12p70 secretion and conveys strong T?cell responses. BMC?Immunol. 2011?Jan?5; 12:2. | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund?Johansen F, Kalina T: High?resolution antibody array analysis of childhood acute leukemia cells. Mol?Cell?Proteomics. 2016?Apr?1; 15(4):1246?61.

Article successfully added.