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CyFlow™ CD2 Purified

CyFlow™ CD2 Purified
Alternative Name: LFA-2
Antigen: CD2
Application: Flow cytometry, Immunoprecipitation
Clonality: monoclonal
Clone: MEM-65
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BU366628

For Research Use Only

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HLDA Workshop HLDA VI—WS Code T 6T-012 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD2 Purified
HLDA WorkshopHLDA VI—WS Code T 6T-012
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman peripheral T cells
Background InformationCD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Drbal K, Hilgert I, Cebecauer M, Angelisova P, Horejsi V: New monoclonal antibodies to human leucocyte surface molecule CD2. Folia Biol (Praha). 1997; 43(6):245‑6. < PMID: 9595269 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass‑supported planar bilayers reconstituted with the immunoglobulin‑related adhesion molecule CD58. J Biol Chem. 1997 Jun 20; 272(25):15782‑8. < PMID: 9188475 > | Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2‑CD58‑Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21; 1(10):649‑58. < PMID: 17168569 > | Espagnolle N, Depoil D, Zaru R, Demeur C, Champagne E, Guiraud M, Valitutti S: CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse. Int Immunol. 2007 Mar; 19(3):239‑48. < PMID: 17220479 > | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci USA. 2007 Mar 20; 104(12):5062‑6. < PMID: 17360380 > | Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21; 13(31):4207‑13. < PMID: 17696249 >