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CyFlow™ CD4 Purified

CyFlow™ CD4 Purified
Alternative Name: Leu3a, T4
Antigen: CD4
Application: Flow cytometry, Functional assays, Immunoprecipitation
Clonality: monoclonal
Clone: MEM-115
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG2a
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CX222689

For Research Use Only

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HLDA Workshop HLDA V—WS Code T T-CD04.09 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD4 Purified
HLDA WorkshopHLDA V—WS Code T T-CD04.09
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent‑resistant T‑cell membrane microdomains. Tissue Antigens. 1999 Jan; 53(1):33‑40. < PMID: 10082429 > | Singer II, Scott S, Kawka DW, Chin J, Daugherty BL, DeMartino JA, DiSalvo J, Gould SL, Lineberger JE, Malkowitz L, Miller MD, Mitnaul L, Siciliano SJ, Staruch MJ, Williams HR, Zweerink HJ, Springer MS: CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells.. J Virol. 2001 Apr; 75(8):3779‑90. < PMID: 11264367 > | Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci USA. 2002 Feb 19; 99(4):2008‑13. < PMID: 11854499 > | Clapham PR, McKnight A: Cell surface receptors, virus entry and tropism of primate lentiviruses. . J Gen Virol. 2002 Aug; 83(8):1809‑29. < PMID: 12124446 > | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17; 198(10):1453‑62. < PMID: 14610046 > | Bosze S, Caccamo N, Majer Z, Mezo G, Dieli F, Hudecz F: In vitro T‑cell immunogenicity of oligopeptides derived from the region 92‑110 of the 16‑kDa protein of Mycobacterium tuberculosis. Biopolymers. 2004; 76(6):467‑76. < PMID: 15386261 >