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CyFlow™ CD9 FITC

CyFlow™ CD9 FITC
Alternative Name: MRP-1 , P24
Antigen: CD9
Application: Flow cytometry
Clonality: monoclonal
Clone: EM-04
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: BL581262

For Research Use Only

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Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD9 FITC
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenPermeabilized murine bone marrow-derived mast cells (BMMC)
Background InformationCD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 6·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Schmidt C, Künemund V, Wintergerst ES, Schmitz B, Schachner M: CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the alpha 6/beta 1 integrin and the neural adhesion molecule L1. J Neurosci Res. 1996 Jan 1; 43(1):11658. < PMID: 8838570 > | Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C: Severely reduced female fertility in CD9‑deficient mice. Science. 2000 Jan 14; 287(5451):319‑21. < PMID: 10634790 > | Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb; 36(1):121‑30. < PMID: 16461932 > | Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci USA. 2007 Jan 2; 104(1):234‑9. < PMID: 17190803 > | Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr; 203(2):377‑86. < PMID: 18799160 > | Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cruz AC, Guzman MC, Nunn MA, Paesen GC, Nuttall PA, Austyn JM: Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses. PLoS Pathog. 2013 Jun 27; 9(6):e1003450. < PMID: 23825947 > | Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial Membrane Vesicles Mediate the Release of Mycobacterium tuberculosis Lipoglycans and Lipoproteins from Infected Macrophages. J Immunol. 2015 Aug 1; 195(3):1044-53. < PMID: 26109643 >