Article successfully added.

CyFlow™ CD11a Biotin

CyFlow™ CD11a Biotin
Alternative Name: LFA-1
Antibody: Yes
Antigen: CD11a
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-25
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CB809014

For Research Use Only

$188.40 CAD*

Price excludes any applicable taxes plus shipping costs

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD11a Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenLeukocytes of a patient suffering from LGL-type leukemia
Background InformationCD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 8.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V: Monoclonal antibodies against human leucocyte antigens (IV): Antibodies against subunits of the LFA‑1 (CD11a/CD18) leucocyte‑adhesion glycoprotein. Folia Biol (Praha). 1990; 36(1):41‑50. < PMID: 1971601 > | Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym‑1 antibody‑dependent cytolysis by neutrophils exposed to granulocyte‑macrophage colony‑stimulating factor: intervention of FcgammaRII (CD32), CD11b‑CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15; 93(10):3505‑11. < PMID: 10233903 > | Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L‑selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18. J Immunol. 1999 Sep 1; 163(5):2891‑901. < PMID: 10453036 > | Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c‑CD18, gp150‑95). J Immunol. 2000 Jun 1; 164(11):5928‑34. < PMID: 10820275 > | Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll‑like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar; 9(2):403‑11. < PMID: 11874886 > | Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein‑Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar; 14(1):34‑9. < PMID: 12799212 > | Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4; 4:3. < PMID: 12659660 > | Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM‑1 antibody‑dependent cytolysis by human neutrophils exposed to GM‑CSF: auto‑regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan; 75(1):99‑105. < PMID: 14525961 > | Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function‑associated antigen 1‑mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule‑1. J Biol Chem. 2005 Aug 5; 280(31):28290‑8. < PMID: 15955822 > | Giblin PA, Lemieux RM: LFA‑1 as a key regulator of immune function: approaches toward the development of LFA‑1‑based therapeutics. Curr Pharm Des. 2006; 12(22):2771‑95. < PMID: 16918410 > | Kellersch B, Kolanus W: Membrane‑proximal signaling events in beta‑2 integrin activation. Results Probl Cell Differ. 2006; 43:245‑57. < PMID: 17068975 > | Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function‑associated antigen‑1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30; 80(24‑25):2320‑4. < PMID: 17289088 > | Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM‑1), but not ICAM‑2 and ‑3, is important for dendritic cell‑mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May; 83(9):4195‑204. < PMID: 19211748 > | Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. eLife. 2015 Dec 9; 4:e10766. < PMID: 26650353 >