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CyFlow™ CD14 Alexa Fluor™ 700

CyFlow™ CD14 Alexa Fluor™ 700
Alternative Name: LPS-R
Antibody: Yes
Antigen: CD14
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-15
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 700
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BC549280

For Research Use Only

$335.58 CAD*

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HLDA Workshop HLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS... more
CyFlow™ CD14 Alexa Fluor™ 700

HLDA WorkshopHLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS Code M MA086 HLDA VI—WS Code M MA94
Quantity100 tests
Volume0.4 mL
ImmunogenA crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
Background InformationCD14 (LPS-R) is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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