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CyFlow™ CD16 Alexa Fluor™ 647

CyFlow™ CD16 Alexa Fluor™ 647
Alternative Name: FcγRIII
Antigen: CD16
Application: Flow cytometry
Clonality: monoclonal
Clone: 3G8
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BR245099

For Research Use Only

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HLDA Workshop HLDA V—WS Code NK80 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD16 Alexa Fluor™ 647
HLDA WorkshopHLDA V—WS Code NK80
Quantity100 tests
Volume0.4 mL
ImmunogenHuman neutrophils
Background InformationCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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