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CyFlow™ CD19 Low Endotoxin

CyFlow™ CD19 Low Endotoxin
Alternative Name: B4
Antigen: CD19
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation
Clonality: monoclonal
Clone: 1D3
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: Low Endotoxin
Isotype: IgG2a
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: BQ572778

For Research Use Only

$153.07 CAD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD19 Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenMouse CD19-transfected cell line
Background InformationCD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self‑renewal of B‑1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan; 26(1):238‑42. < PMID: 8566073 > | Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1; 157(1):48‑56. < PMID: 8683154 > | Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement‑tagged antigens. J Immunol. 2001 Jul 1; 167(1):163‑72. < PMID: 11418645 > | Inabe K, Kurosaki T: Tyrosine phosphorylation of B‑cell adaptor for phosphoinositide 3‑kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15; 99(2):584‑9. < PMID: 11781242 > | Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15; 171(8):4062‑72. < PMID: 14530327 >