CyFlow™ CD11a Biotin
| Alternative Name: | LFA-1 |
| Antibody: | Yes |
| Antigen: | CD11a |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-25 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CB809014 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
| Background Information | CD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 8.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V: Monoclonal antibodies against human leucocyte antigens (IV): Antibodies against subunits of the LFA‑1 (CD11a/CD18) leucocyte‑adhesion glycoprotein. Folia Biol (Praha). 1990; 36(1):41‑50. < PMID: 1971601 > | Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym‑1 antibody‑dependent cytolysis by neutrophils exposed to granulocyte‑macrophage colony‑stimulating factor: intervention of FcgammaRII (CD32), CD11b‑CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15; 93(10):3505‑11. < PMID: 10233903 > | Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L‑selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18. J Immunol. 1999 Sep 1; 163(5):2891‑901. < PMID: 10453036 > | Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c‑CD18, gp150‑95). J Immunol. 2000 Jun 1; 164(11):5928‑34. < PMID: 10820275 > | Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll‑like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar; 9(2):403‑11. < PMID: 11874886 > | Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein‑Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar; 14(1):34‑9. < PMID: 12799212 > | Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4; 4:3. < PMID: 12659660 > | Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM‑1 antibody‑dependent cytolysis by human neutrophils exposed to GM‑CSF: auto‑regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan; 75(1):99‑105. < PMID: 14525961 > | Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function‑associated antigen 1‑mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule‑1. J Biol Chem. 2005 Aug 5; 280(31):28290‑8. < PMID: 15955822 > | Giblin PA, Lemieux RM: LFA‑1 as a key regulator of immune function: approaches toward the development of LFA‑1‑based therapeutics. Curr Pharm Des. 2006; 12(22):2771‑95. < PMID: 16918410 > | Kellersch B, Kolanus W: Membrane‑proximal signaling events in beta‑2 integrin activation. Results Probl Cell Differ. 2006; 43:245‑57. < PMID: 17068975 > | Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function‑associated antigen‑1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30; 80(24‑25):2320‑4. < PMID: 17289088 > | Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM‑1), but not ICAM‑2 and ‑3, is important for dendritic cell‑mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May; 83(9):4195‑204. < PMID: 19211748 > | Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. eLife. 2015 Dec 9; 4:e10766. < PMID: 26650353 >