CyFlow™ CD11b APC
| Alternative Name: | Mac-1 |
| Antibody: | Yes |
| Antigen: | CD11b |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | M1/70 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping, MHC |
| Format/Fluorochrome: | APC |
| Isotype: | IgG2b |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Rat |
| Target Species: | Human, Mouse, Non-Human Primates, Rabbit |
| Product number: | BR751578 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,5 |
| Quantity | 0.1 mg |
| Volume | 0.2 mL |
| Immunogen | B10 mouse spleen cells enriched for T cells |
| Background Information | CD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
| Usage | The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug; 8(8):539‑51. < PMID: 81133 > | Ault KA, Springer TA: Cross‑reaction of a rat‑anti‑mouse phagocyte‑specific monoclonal antibody (anti‑Mac‑{01} with human monocytes and natural killer cells. J Immunol. 1981 Jan; 126(1):359‑64. < PMID: 7451976 > | Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha‑ and beta‑subunits of two related mouse glycoproteins involved in cell interactions, LFA‑1 and Mac‑1. J Exp Med. 1983 Aug 1; 158(2):586‑602. < PMID: 6193226 > | Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T‑cell subsets and other leukocytes in paraffin‑embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar; 43(3):313‑20. < PMID: 7868861 > | Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor‑specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci USA. 2000 Mar 14; 97(6):2697‑702. < PMID: 10706628 > | Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon‑injured arteries. Arterioscler Thromb Vasc Biol. 2000 Dec; 20(12):2553‑8. < PMID: 11116052 > | Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft‑versus‑host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15; 168(6):3088‑98. < PMID: 11884483 > | Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood‑borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J Appl Physiol. 2003 Sep; 95(3):969‑76. < PMID: 12730143 > | Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J Immunol. 2006 Nov 15; 177(10):6974‑82. < PMID: 17082612 > | Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM‑3 grabbing nonintegrin‑related 1 (SIGNR{01} and complement receptor type 3 (CR3) in the uptake of oligomannose‑coated liposomes by macrophages. Glycobiology. 2009 Mar; 19(3):258‑66. < PMID: 19029201 >