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CyFlow™ CD15 FITC

CyFlow™ CD15 FITC
Alternative Name: Lewis X
Antibody: Yes
Antigen: CD15
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-158
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Blood group antigens, Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgM
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CM349973

For Research Use Only

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 2.0 mL Immunogen Human granulocytes Background... more
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CyFlow™ CD15 FITC

Quantity100 tests
Volume2.0 mL
ImmunogenHuman granulocytes
Background InformationCD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov; 24(11):771‑6. < PMID: 1362193 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non‑sialyl‑CD15 antigens on Hodgkin‑Reed‑Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep; 39(1‑2):185‑94. < PMID: 10975398 > | Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1; 406(2):333‑41. < PMID: 17497959 >

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