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CyFlow™ CD16 Purified

CyFlow™ CD16 Purified
Alternative Name: FcγRIII
Antibody: Yes
Antigen: CD16
Application: Flow cytometry, Immunoprecipitation
Clonality: monoclonal
Clone: LNK16
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: AB691965

For Research Use Only

$141.30 CAD*

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD16 Purified

Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenNormal human peripheral blood granulocytes
Background InformationCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III‑A and III‑B genes: Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20; 270(3):1350‑61. < PMID: 7836402 > | Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies: Implications for ligand binding. J Immunol. 1996 Aug 15; 157(4):1576‑81. < PMID: 8759741 > | Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross‑linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15; 159(8):3940‑8. < PMID: 9378982 > | Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4; 4:3. < PMID: 12659660 > | Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE‑mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15; 170(6):3054‑8. < PMID: 12626560 > | Boyle JJ: Human macrophages kill human mesangial cells by Fas‑L‑induced apoptosis when triggered by antibody via CD16. Clin Exp Immunol. 2004 Sep; 137(3):529‑37. < PMID: 15320902 > | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15; 106(10):3567‑74. < PMID: 16046525 > | Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC Immunol. 2011 Jan 5; 12:2. < PMID: 21208424 >

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