CyFlow™ CD103 PE
| Alternative Name: | HML-1 , Integrin αE-subunit |
| Antibody: | Yes |
| Antigen: | CD103 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | Ber-ACT8 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | AX666438 |
For Research Use Only
| HLDA Workshop | HLDA V—WS Code A067 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | HTLV 1-induced human T cell line MAPS16 |
| Background Information | CD103 (Integrin αE) is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-β stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin β7 subunit to form αE/β7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E‑cadherin in immunotherapy?. Br J Cancer. 2002 Oct 21; 87(9):1034‑41. < PMID: 12434297 > | Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct; 77(19):10456‑67. < PMID: 12970431 > | Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T‑cell involvement in humoral immunity. Blood. 2003 Nov 15; 102(10):3693‑701. < PMID: 12881309 > | León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V‑coated magnetic beads. Lab Invest. 2004 Jun; 84(6):804‑9. < PMID: 15154017 > | Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E‑selectin, cutaneous lymphocyte‑associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul; 13(7):426‑34. < PMID: 15217363 > | Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul; 84(1):311‑8. < PMID: 18451325 > | Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1; 205(9):2139‑49. < PMID: 18710932 > | Kost CB, Holden JT, Mann KP: Marginal zone B‑cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep; 74(5):282‑6. < PMID: 18500740 > | Kost CB, Holden JT, Mann KP: Marginal zone B‑cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep; 74(5):282‑6. < PMID: 18500740 > | Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF‑beta‑producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15; 183(10):6346‑58. < PMID: 19841178 >