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CyFlow™ CD107a Alexa Fluor™ 488

CyFlow™ CD107a Alexa Fluor™ 488
Alternative Name: LAMP-1
Antibody: Yes
Antigen: CD107a
Application: Flow cytometry
Clonality: monoclonal
Clone: H4A3
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse, Non-Human Primates
Product number: CG942258

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Human peripheral blood mononuclear cells... more
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CyFlow™ CD107a Alexa Fluor™ 488
Quantity100 tests
Volume0.4 mL
ImmunogenHuman peripheral blood mononuclear cells (PBMC)
Background InformationCD107a (LAMP-1; lysosome-associated membrane protein-1), together with CD107b (LAMP-2), is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.02% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan; 268(1):360‑78. < PMID: 2912382 > | Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome‑associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug; 159(2):449‑55. < PMID: 11485903 > | Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T‑cell receptor plus the Toll‑Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug; 74(8):4505‑11. < PMID: 16861636 > | Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer‑cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13‑4 defect and discriminates between genetic subtypesof the disease. Blood. 2006 Oct 1; 108(7):2316‑23. < PMID: 16778144 > | Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule‑1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1; 67(3):1317‑25. < PMID: 17283169 > | Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV‑1‑infected autologous CD4 primary T cells: requirement for IFN‑mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15; 179(4):2097‑104. < PMID: 17675468 > | Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza‑specific CD8+ T‑cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1; 112(9):3671‑8. < PMID: 18713944 > | Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May; 84(9):4148‑57. < PMID: 20164232 >

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