CyFlow™ CD2 PE-Cy7
| Alternative Name: | LFA-2 |
| Antigen: | CD2 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | LT2 |
| Emission Maximum: | 785 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE-Cy7 |
| Isotype: | IgG2b |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BZ621517 |
CE IVD
| HLDA Workshop | HLDA VI—WS Code T 6T-008 |
| Concentration Unit | µg/mL |
| Concentration | 10 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Normal human blood lymphocytes |
| Background Information | CD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. |
| Antigen Distribution | CD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Filatov AV, Bachurin PS, Markova NA, Surnakova NE: A panel of monoclonal antibodies against human lymphocyte antigens. Eksp Onkol. 1989; 11(2):28‑32. < PMID: 2737128 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass‑supported planar bilayers reconstituted with the immunoglobulin‑related adhesion molecule CD58. J Biol Chem. 1997 Jun 20; 272(25):15782‑8. < PMID: 9188475 > | Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am J Physiol. 1998 Dec; 275(6‑2):R1725‑34. < PMID: 9843861 > | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15; 106(10):3567‑74. < PMID: 16046525 > | Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2‑CD58‑Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21; 1(10):649‑58. < PMID: 17168569 > | Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21; 13(31):4207‑13. < PMID: 17696249 >