CyFlow™ CD3 Pacific Blue™
| Alternative Name: | Leu4, T3 |
| Antigen: | CD3 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | UCHT1 |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Pacific Blue™ |
| Isotype: | IgG1 |
| Laser: | Violet |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AJ544819 |
CE IVD
| HLDA Workshop | HLDA I—WS Code T 3 HLDA III—WS Code T 126 HLDA III—WS Code T 471 HLDA VI—WS Code T 6T-CD3.1 |
| Concentration Unit | µg/mL |
| Concentration | 100 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human thymocytes followed by Sezary T cells |
| Background Information | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
| Antigen Distribution | CD3 (T3, Leu4) is expressed on peripheral blood lymphocytes, thymocytes and Purkinje cells in the cerebellum; it is lost on B or NK cells. CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3δ, CD3ε and CD3ζ. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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