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CyFlow™ CD5 PE-Cy7

CyFlow™ CD5 PE-Cy7
Alternative Name: Leu-1, T1
Antigen: CD5
Application: Flow cytometry
Clonality: monoclonal
Clone: L17F12
Emission Maximum: 785 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE-Cy7
Isotype: IgG2a
Laser: Blue , Green, Yellow
Regulatory Status: CE IVD
Source Species: Mouse
Target Species: Human
Product number: AZ519916

CE IVD

HLDA Workshop HLDA II Concentration Unit µg/mL Concentration 20 Quantity 100... more
CyFlow™ CD5 PE-Cy7

HLDA WorkshopHLDA II
Concentration Unitµg/mL
Concentration20
Quantity100 tests
Volume1.0 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Antigen DistributionCD5 (T1, Leu-1) is a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
UsageCE IVD usage Placeholder
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci USA. 1981 Mar; 78(3):1791‑5. < PMID: 7015346 > | Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T‑cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990 Jan 1; 75(1):166‑73. < PMID: 1688495 > | McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N‑terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein Eng. 1998 Oct; 11(10):847‑53. < PMID: 9862202 > | Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec; 116(6):893‑7. < PMID: 11764079 > | Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch Pathol Lab Med. 2007 May; 131(5):748‑54. < PMID: 17488160 >