CyFlow™ CD8 Pacific Blue™
| Antigen: | CD8 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-31 |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Pacific Blue™ |
| Isotype: | IgG2a |
| Laser: | Violet |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AG365256 |
CE IVD
| HLDA Workshop | HLDA III—WS Code T 575 |
| Concentration Unit | µg/mL |
| Concentration | 10 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Crude thymus membrane fraction |
| Background Information | The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
| Antigen Distribution | CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia Biol (Praha). 1986; 32(1):12‑25. < PMID: 2422063 > | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia Biol (Praha). 1988; 34(1):23‑34. < PMID: 2968928 > | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17; 198(10):1453‑62. < PMID: 14610046 > | Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15; 177(6):3930‑8. < PMID: 16951356 > | Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1; 178(7):4402‑10. < PMID: 17371997 > | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May; 19(5):675‑84. < PMID: 17446208 > | Pang DJ, Hayday AC, Bijlmakers MJ: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4; 282(18):13884‑94. < PMID: 17341584 > | van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8‑driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21; 249(2):395‑408. < PMID: 17869274 > | Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J Biomed Biotechnol. 2010; 2010:841451. < PMID: 20339516 > | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund‐Johansen F, Kalina T: High‐resolution antibody array analysis of childhood acute leukemia cells. Mol Cell Proteomics. 2016 Apr 1; 15(4):1246‐61. < PMID: 26785729 >