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CyFlow™ CD16 Pacific Orange™

CyFlow™ CD16 Pacific Orange™
Alternative Name: FcγRIII
Antigen: CD16
Application: Flow cytometry
Clonality: monoclonal
Clone: 3G8
Emission Maximum: 551 nm
Excitation Maximum: 400 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Pacific Orange™
Isotype: IgG1
Laser: Violet
Regulatory Status: CE IVD
Source Species: Mouse
Target Species: Human
Product number: AK192276

CE IVD

HLDA Workshop HLDA V—WS Code NK80 Concentration Unit µg/mL Concentration 220... more
CyFlow™ CD16 Pacific Orange™

HLDA WorkshopHLDA V—WS Code NK80
Concentration Unitµg/mL
Concentration220
Quantity100 tests
Volume1.0 mL
ImmunogenHuman neutrophils
Background InformationCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Antigen DistributionCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa) expressed on NK-cells, monocytes and macrophages and CD16b (FcγRIIIB; 48 kDa) mainly expressed on neutrophils.
UsageCE IVD usage Placeholder
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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