CyFlow™ CD33 FITC
Alternative Name: | p67 , SIGLECT-3 |
Antigen: | CD33 |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | WM53 |
Emission Maximum: | 518 nm |
Excitation Maximum: | 490 to 495 nm |
Field of Interest: | Immunophenotyping |
Format/Fluorochrome: | FITC |
Isotype: | IgG1 |
Laser: | Blue |
Regulatory Status: | CE IVD |
Source Species: | Mouse |
Target Species: | Human |
Product number: | BG689606 |
CE IVD
HLDA Workshop | HLDA IV—WS Code M-505 |
Concentration Unit | µg/mL |
Concentration | 20 |
Quantity | 100 tests |
Volume | 1.0 mL |
Immunogen | Human Acute Myelogenous Leukemia (AML) cells |
Background Information | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
Antigen Distribution | CD33 (p67, SIGLECT-3) is a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is lost on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. |
Usage | CE IVD usage Placeholder |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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