CyFlow™ CD33 PE
| Alternative Name: | p67 , SIGLECT-3 |
| Antigen: | CD33 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | WM53 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BH256785 |
CE IVD
| HLDA Workshop | HLDA IV—WS Code M-505 |
| Concentration Unit | µg/mL |
| Concentration | 20 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human Acute Myelogenous Leukemia (AML) cells |
| Background Information | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| Antigen Distribution | CD33 (p67, SIGLECT-3) is a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is lost on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid‑differentiation antigen, 'gp67' (CD‑33). Dis Markers. 1987 Dec; 5(4):215‑25. < PMID: 3502990 > | Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160, 95, gp150, gp67) investigation of epitopic heterogeneity and non‑haemopoietic distributionusing panels of monoclonal antibodies belonging to CD‑11b, CD‑13 and CD‑33. Br J Haematol. 1988 Jun; 69(2):163‑71. < PMID: 2455535 > | Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br J Haematol. 1989 Aug; 72(4):512‑8. < PMID: 2673329 > | Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia‑associated antigen, JL1, in bone marrow and thymus. Am J Pathol. 2001 Apr; 158(4):1473‑80. < PMID: 11290565 > | Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM‑1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci USA. 2001 May 8; 98(10):5764‑9. < PMID: 11320212 > | Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell‑derived factor 1/CXCR4 signaling is critical for early human T‑cell development. Blood. 2002 Jan 15; 99(2):546‑54. < PMID: 11781237 > | Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells‑1 are underrepresented in the human intestine. J Immunol. 2005 Jan 1; 174(1):517‑24. < PMID: 15611278 > | Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid‑associated marker CD33 isnot an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005 Feb 1; 105(3):1256‑64. < PMID: 15388576 > | Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow‑derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun; 26(12):1196‑204. < PMID: 15734770 > | Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC‑3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006 Jan; 79(1):46‑58. < PMID: 16380601 >