CyFlow™ CD33 APC
| Alternative Name: | p67 , SIGLECT-3 |
| Antigen: | CD33 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | WM53 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BB544350 |
CE IVD
| HLDA Workshop | HLDA IV—WS Code M-505 |
| Concentration Unit | µg/mL |
| Concentration | 15 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human Acute Myelogenous Leukemia (AML) cells |
| Background Information | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| Antigen Distribution | CD33 (p67, SIGLECT-3) is a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is lost on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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