CyFlow™ CD45 APC
| Alternative Name: | B220, LCA , T200 |
| Antigen: | CD45 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 2D1 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CY109146 |
CE IVD
| HLDA Workshop | HLDA III – WS Code 792 |
| Concentration Unit | µg/mL |
| Concentration | 60 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human peripheral blood mononuclear cells (PBMC) |
| Background Information | CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. |
| Antigen Distribution | CD45 (LCA, T200, B220) is a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS‑induced arachidonic acid metabolism in macrophages. Biochim Biophys Acta. 2000 Feb 28; 1495(3):212‑22. < PMID: 10699460 > | Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4(+)‑T‑cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin Diagn Lab Immunol. 2002 Sep; 9(5):1085‑94. < PMID: 12204964 > | Wellhausen SR, Slone SP, Miller JJ: Clone‑specific anti‑CD45 blocking factor in patient plasma. Cytometry B Clin Cytom. 2007 Sep; 72(5):423‑6. < PMID: 17311353 > | Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti‑CCR4 monoclonal antibody exercises potent ADCC‑mediated antitumor effect in the novel tumor‑bearing humanized NOD/Shi‑scid, IL‑2Rgamma(null) mouse model. Cancer Immunol Immunother. 2009 Aug; 58(8):1195‑206. < PMID: 19048251 > | Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Hematol. 2010 Feb; 32(1‑1):e40‑8. < PMID: 20088999 > | Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS One. 2013; 8(3):e58565. < PMID: 23555586 >