CyFlow™ CD71 Alexa Fluor™ 700
| Alternative Name: | T9 , TFR, TRFR |
| Antigen: | CD71 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-75 |
| Emission Maximum: | 723 nm |
| Excitation Maximum: | 702 nm |
| Field of Interest: | Endocytosis, Immunophenotyping |
| Format/Fluorochrome: | Alexa Fluor™ 700 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CT702956 |
CE IVD
| HLDA Workshop | HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 |
| Concentration Unit | µg/mL |
| Concentration | 100 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | NALM-6 human pre-B cell line |
| Background Information | CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. |
| Antigen Distribution | CD71 (T9, TFR, TRFR) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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