CyFlow™ CD200 APC
| Alternative Name: | MOX1, MOX2, MRC, OX-2 |
| Antigen: | CD200 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | OX-104 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping, Neurobiology |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | CE IVD |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CS291900 |
CE IVD
| HLDA Workshop | HLDA VII—WS Code 70655 |
| Concentration Unit | µg/mL |
| Concentration | 35 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human CD200 |
| Background Information | CD200 (OX-2, MRC, MOX1, MOX2) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-α and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization. |
| Antigen Distribution | CD200 (OX-2, MRC, MOX1, MOX2) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-α and IL-13 secretion and overall attenuation of the activation status of lymphocytes. |
| Usage | CE IVD usage Placeholder |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down‑regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul; 78(14):7667‑76. < PMID: 15220441 > | Shiratori I, Yamaguchi M, Suzukawa M, Yamamoto K, Lanier LL, Saito T, Arase H: Down‑regulation of basophil function by human CD200 and human herpesvirus‑8 CD200. J Immunol. 2005 Oct 1; 175(7):4441‑9. < PMID: 16177086 > | Sopp P, Werling D, Baldwin C: Cross‑reactivity of mAbs to human CD antigens with cells from cattle.. Vet Immunol Immunopathol. 2007 Sep 15; 119(1‑2):106‑14. < PMID: 17675249 > | Meuth SG, Simon OJ, Grimm A, Melzer N, Herrmann AM, Spitzer P, Landgraf P, Wiendl H: CNS inflammation and neuronal degeneration is aggravated by impaired CD200‑CD200R‑mediated macrophage silencing. J Neuroimmunol. 2008 Feb; 194(1‑2):62‑9. < PMID: 18164423 > | Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G: Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200‑rich and CD34‑positive hair follicle progenitor cells. J Clin Invest. 2011 Feb; 121(2):613‑22. < PMID: 21206086 > | Li Y, Zhao LD, Tong LS, Qian SN, Ren Y, Zhang L, Ding X, Chen Y, Wang YX, Zhang W, Zeng XF, Zhang FC, Tang FL, Zhang X, Ba DN, He W, Cao XT, Lipsky PE: Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T‑cell responsiveness and dendritic cell activity. Arthritis Res Ther. 2012 May 23; 14(3):R123. < PMID: 22621248 > | Colmont CS, Benketah A, Reed SH, Hawk NV, Telford WG, Ohyama M, Udey MC, Yee CL, Vogel JC, Patel GK: CD200‑expressing human basal cell carcinoma cells initiate tumor growth. Proc Natl Acad Sci USA. 2013 Jan 22; 110(4):1434‑9. < PMID: 23292936 >