CyStain™ BacCount Viable

Reagent kit intended for the unspecific enumeration of all bacteria (Total Cell Count) and the enumeration of viable bacteria (Viable Cell Count) in water samples. The CyStain BacCount Viable kit discriminates between live and dead bacteria, which is important to monitor successful inactivation of harmful bacteria in drinking water. Designed for the quality control of water from different sources (ground water, spring water or surface water), but can also be used to control water used in industrial applications. CyStain™ Green is a membrane permeable dye that unspecifically stains all bacteria in water samples, emitting green fluorescence. Both live and dead cells will be stained. CyStain™ Red is not membrane permeable and stains nucleic acids. It is used as an indicator of dead cells. A flow cytometer with a blue laser (488 nm) and detectors for forward scatter, side scatter, green fluorescence and red fluorescence is required. The Sysmex CyFlow™ Cube 6 V2m is recommended.                                                                                                      

Laser: Blue

Fluorescent probe: CyStain Green, CyStain Red                                                                      

Kit components:

• 5 aliquots (40µL each) of CyStain Green (x1000 concentrated in DMSO)
• 5 aliquots (400µL each) of CyStain Red
• 29 mL CyStain Dilution Buffer
• Quantity 200 tests (manual) 1000 tests (autoloader)